This program is aimed at preventing post-transplantation organ failure by modern drug delivery strategy. Based on NHC Key Laboratory of Combined Multi-organ Transplantation, we have established a small molecule prodrug platform to invent self-assembly nano-medicines for tissue-specific delivery with optimized pharmacokinetics and enhanced bioavailability. Then, we have applied this self-assembly pro-drug strategy in the combat of post-transplantation organ failure, and have proposed multiple working models of modern nano-medicines.

Target participants: graduate student, post-doctors, research scientists.

This program is multi-disciplinary, consisting of pharmacy, transplantation immunology, chemistry, and nanobiology. Classical animal models, including MHC-mismatched allotransplantation model and ischemia-reperfusion model, are utilized to evaluate the safety, stability and therapeutic efficacy of self-assembly nano-medicines. Members of this projects have published more than 40 papers in top journals such as Nature Communications, PNAS, Am J Transplantation, and eBiomedicine.

Trainees would acquire the following knowledge and lay the foundation for the research of transplantation immunology and immune-modulatory medicine after this program:

· Basic knowledge of transplantation immunology;

· Standard protocols of establishing classical animal models for the research of transplantation immunology;

· Principles for the design and synthesis of self-assembly nano-medicines;

· Laboratory techniques for the extraction, isolation and analysis of immunocyte, including spectral flow cytometry analysis.

1 week

Research area:

· Transplantation immunology

· Acute/chronic allograft rejection

· HCC recurrence after liver transplantation

· Immune-modulatory medicine

· Nano-medicine

Project title:

Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences (2023-PT320-02), Mechanism study on graft dysfunction after liver transplantation and innovative prevention strategies. 2023.09-2026.08.

Graduate students:

Education on biology, pharmacology or medicine

Researcher fellows:

Education on biology, pharmacology or medicine

Postdocs:

Education on biology, pharmacology or medicine

Daily Training Schedule:

Monday:

Time duration: 9:00 am to 4:00 pm.

Topics and key activities:

1. Theoretical courses for transplantation immunology and its research models;

2. Theoretical courses about the design, synthesis and assembling of prodrug.

Format: lectures.

Tuesday:

Time duration: 9:00 am to 4:00 pm.

Topics and key activities: Protocols of establishing skin transplantation model.

Format: Lecture and hands-on training.

Wednesday:

Time duration: 9:00 am to 4:00 pm.

Topics and key activities: Protocols of establishing model of ischemia-reperfusion injury .

Format: Lecture and hands-on training.

Thursday-Saturday:

Time duration: 9:00 am to 4:00 pm for each day.

Topics and key activities: The synthesis of small molecular prodrugs and the isolation and analysis of immunocytes.

Format: Lecture and hands-on training.

No examination is required, and a certificate would be issued at the end of this training program. Online technical support would be provided for trainees' future research activity.

Participants could directly contact the Program Coordinator for further inquiries.

The program is free of charge.