Central Laboratory

Overview

The Central Laboratory of the First Affiliated Hospital of Zhejiang University School of Medicine was established in October 2011. It is an open platform integrating scientific research, teaching, clinical practice and community service altogether. The goal of the central laboratory is to provide advanced technology, with a variety of platforms, and high-quality service for investigators in all departments of the hospital, to stipulate academic environment for uninhibited exploration and team cooperation, and to improve the scientific research of the hospital, culminating in a first-class scientific research center.

Service

The central laboratory is capable of carrying out most routine experiments. In addition to molecular and cell culture, the central laboratory also has platforms of flow cytometry, imaging, quantitative PCR, gene polymorphism detection and liquid biopsy centered on exosomes. Instruments in the Laboratory include BD Calibur flow cytometry, Odyssey CLX dual color infrared laser image system, Leica DM2000 positive fluorescence microscope, Nikon Ti-s inverted fluorescence microscope, ABI Quantstudio Q5 fluorescent quantitative PCR instrument, ABI 7500 fluorescence quantitative PCR instrument, chef-mapper-xa pulse field electrophoresis system, DCode gene mutation detection system, Nanosight NS300 particle tracking analysis system and NanoFCM Nanoflow detection system. Of note, the liquid biopsy is with the most advanced equipment for exosome research, providing scientific and technical support for investigations focused on exosomes.

Research

The central laboratory currently offers an open platform including flow cytometry, imaging, fluorescence quantitative PCR, and exosome investigations related to liquid biopsy.

The research work of the central laboratory is robustly supported by grants from national, provincial and ministerial levels of scientific projects, including 863, main and sub projects, general and youth projects of National Natural Science Foundation of China (NSFC), youth projects of Provincial Natural Science Foundation, and Science and Technology projects of Provincial Department of Health.

Education

Under the training and management of the central laboratory members, hundreds of doctoral and postgraduate students from the departments of respiratory medicine, gastroenterology, cardiovascular medicine, anesthesiology, radiotherapy, ophthalmology, orthopedics, urology, oncology, mental health, neurology, and neurosurgery have effectively carried out scientific research in the central laboratory and successfully completed their studies.

Department Director

Jing Zhang,Endowed Professor, Doctoral Supervisor.

Research on the pathogenesis of neurodegenerative disorders, including Alzheimer's disease (AD) and Parkinson's disease (PD), and translational medicine, focusing on molecular markers of early diagnosis and tracking of neurodegenerative disorders.

Prof. Zhang was awarded the international AD biomarker Research and Development Award by the Journal of Alzheimer's disease. He initiated several research areas, e.g. from mass spectrometry discovery to independent verification, with various state-of-the-art assays, and led one of the first teams to confirm the clinical value of cerebrospinal fluid α-synuclein (α-syn), phosphorylated α-syn, Aβ, tau in early diagnosis and differential diagnosis of PD. Prof. Zhang also participated in the taskforce of international standardization of body fluid biomarkers and inter laboratory quality control studies organized by NIH and MJFF PD biomarker alliance. Prof Zhang also pioneered the technology of capturing brain-derived exosomes in peripheral fluids, especially blood and saliva. The application of α-syn and tau in plasma exosomes has been granted an international patent. This technology breaks through the technical bottleneck of brain-derived molecular genomics research from peripheral fluids and creates a new perspective on noninvasive early diagnosis of brain diseases through peripheral body fluids.

About 250 SCI articles have been published, including world top journals such as Lancet Neurology (IF:28.75), Acta Neuropathologica (IF:18.17), Science Translational Medicine (IF:17.16), Alzheimer’s & Dementia (IF:14.42), Journal of Clinical Investigation (IF:12.28), Brain (IF:11.81), PNAS (IF:9.50), Annals of Neurology (IF:10.24), Neurology (IF:8.68), Movement Disorders (IF:8.06). The total number of references exceeded 17000, h-index: 70, I10 index: 195.